Preparation of Diphenylisoxazoline by a Dipolar Cycloadditio

Preparation of Diphenylisoxazoline by a Dipolar CycloadditioAimsThe aims of this experiment were to synthesise a diphenylisoxazoline by a 1,3- dipolar cycloaddition reaction this involved the preparation of an oxime which was oxidised to form a rather unstable nitrile oxide which was trapped in situ with an alkene to devolve an isoxazoline. to fully characterise both, the intermediate oxime and the final isoxazoline, with Infra-Red and Proton NMR spectra.data-basedPreparation of benzaldehyde oximeIn a fume cupboard, sodium hydroxide (3.5g) was dissolve in water (30mL) in a 100mL conical flask containing a magnetic tiltrer bar. The solution was then allowed to cool down to ambient temperature and benzaldehyde (0.5mL) was added followed by hydroxylamine hydrochloride (0.5g). The stirrer was set to a maximum potency to allow for vigorous stiring for about 5 minutes. The conical flask was stopped at this stage.After 5 minutes, the stopper was removed from the flask and and portions o f benzaldehyde (0.5mL) and hydroxylamine hydrochloride (0.5g) were added. This sequence was repeated until all the benzaldehyde (total 5.1mL) and hydroxylamine hydrochloride (total 4.2g) were consumed.The reaction categorization warmed up and the solution became homogeneous indicates complete expending of benzaldehyde.With the aid of a broad-range pH indicator, the reaction mixture was neutralised with glacial acetic acid (1.6mL). At this stage a some drops of water were added to attend dissolving e real sodium acetate precipitate formed. The solution was then allowed to cool and the organic material (top layer) extracted with diethyl ether (2 x 30mL) to a 100mL beaker. A hardly a(prenominal) spatulas of magnesium sulphate were added to the beaker to dry the organic extracts. The mixture was filtered off into a round-bottomed flask and the solvent removed on a rotary evaporator.The yield and the IR spectrum of the oil were recorded.1,3-dipolar cycloaddition reactionAgain in a fume cupboard, vinylbenzene (2.9mL) and triethylamine (0.3mL) were dissolved in dichloromethane (15mL) in a 100 mL conical flask. Sodium hypochloride solution (25mL, ca. 10% lendable chlorine) was added whilst stirring with the aid of a magnetic stirrer bar already in the flask. The flask was dictated into an ice bath and the oily oxime (2.5g) was added dropwise with the aid of a Pasteur pipette over a period of 15 minutes. Once addition was completed, the reaction mixture was allowed to stir in the ice bath for a further period of 45 minutes.The whole reaction mixture was transferred to a separating funnel where it was allowed to stand for a few minutes before the lower organic phase was extracted. Afterwards, the remaining aqueous phase was extracted with further dichloromethane (15mL) and both organic extracts combined and dried over magnesium sulphate (a few spatulas as required).The mixture was filtered into a round-bottomed flask, to remove the magnesium sulphate. The flas k was placed onto a rotary evaporator to remove any remaining solvent. The weight of the crude product was recorded and the same recrystallised from ethanol.An IR spectrum was run through the pure product and the yield recorded.ResultsPercentage yield ill-treat 1 Preparation of Benzaldehyde OximeThe first step of this experiment was to synthesise the benzaldehyde oxime. The reaction scheme for this synthesis is as followsStoichiometric ratio 11Benzaldehyde use = 5.1mL density benzaldehyde = 1.0415 gml-1, mass = 5.31g (3 S.F.)Molecular mass = 106.12 gmol-1, therefrom n. of moles = (3 S.F.)NH2OHHCl used = 4.2g Molecular mass = 69.5 gmol.1, hence n. of moles =NaOH used = 3.5g Molecular mass = 40 gmol.1, hence n. of moles =Experimental ratioStoichiometric ratio 11, hence benzaldehyde is the limiting reagent.N. of moles of benzaldehyde = n. of moles of benzaldehyde oximeBenzaldehyde oxime yield = 4.43g Molecular mass = 121.139 gmol.1, thus n. of moles =Step 2 1,3-dipolar cycloadditio n reactionThe preparation of the diphenylisoxazoline by a 1,3-dipolar cycloaddition follows the following reaction schemeBenzaldehyde oxime used = 2.50g Molecular mass = 121.14 gmol-1, thereof n. of moles =Styrene used = 2.90mL = 2.64g Molecular mass = 104.15 gmol-1, hence n. of moles =NaOCl (ca. 10% available Cl) used = 25 mL density NaOCl = 1.206 gmL-1, hence 30.15g used. Molecular mass = 74.5 gmol-1, therefore n. of moles =C6H15N used = 0.3 mL density C6H15N = 0.726 gcm-3, hence 0.218g used. Molecular mass = 101.19 gmol-1, therefore n. of moles =Stoichiometric ratio of benzaldehyde oxime reacting with styrene is of 11Benzaldehyde oxime is the limiting reagentN. of moles of benzaldehyde oxime = n. of moles of diphenylisoxazolineYield of diphenylisoxazoline = 1.00g molecular mass = 223.270 gmol-1, thus n. of moles =Overall % yieldSpectroscopic data pairing ConstantsH8 at CD 2J8,7 = 16.4 Hz , 3J8,6 = 8.4 HzH7 at CD 2J7,8 = 16.4 Hz , 3J7,6 = 11.2 HzH6 at CE 3J6,7 = 11.2 Hz, 3J6 ,8 = 8.4 HzInfra-Red SpectraBenzaldehyde OximeO-H- (stretch) 3500-3100 cm-1, broad peakC=N- 1650 cm-1sp3 C-H 3100-2750 (including aldehyde sp3 C-H)C=C aromatic 1450-1500 cm-1 (3 medium peaks).N-OH 960 cm-13,5-Diphenyl-2-isoxazolineN-O 920 cm-1 (sharp, medium)sp3 (phenyl) C-H and sp2 (azoline) C-H (stretch) 2800-3200 cm-1C-O 900 cm-1 (sharp, strong)C=C aromatic 1450-1500 cm-1C=N- 1650 cm-1 (sharp, weak)Other DataBefore the organic phase was extracted, during the synthesis of benzaldehyde oxime, the reaction mixture was neutralized with glacial acetic acid, as per stated in the experimental session of this paper.The amount of acid necessary was calculated as follows, in golf club to ensure an accurate amount of acid added to the reaction mixtureN. of moles NaOH =NH2OHHCl n. of moles =Excess of NaOH used = n. of moles of CH3CO2H needed.Molecular mass CH3CO2H = 60.1 gmol-1 , hence mass of CH3CO2H = 1.63g.Density of CH3CO2H = 1.049 g/mL, therefore volume needed 1.60 mLDiscussionPr eparation of benzaldehyde oximeThe first step of this experiment Preparation of benzaldehyde oxime, is a simple condensation reaction between an aldehyde (benzaldehyde) and hydroxylamine.The benzaldehyde oxime watchful was clear oil with a relatively good % yield (73%).The comparison between the infrared spectra of the benzaldehyde oxime in the literature and the single recorded for this experiment (attached in the end of this paper) clearly indicates the successful preparation of the same.The Nujol peaks are shown more strongly in the prepared spectra, but nevertheless it proves a clear way of identifying the working(a) groups of this compound.M.p. ranges were not measured, and therefore even though the IR spectrum correlates to the actual oximes, its purity should be treated as questionable.1,3-dipolar Cycloaddition ReactionIn this second step of the experiment, the syn-benzaldehyde oxime produced undergoes hypochlorite oxidisation to form the 1,3-dipolar benzonitrile oxide wh ich then reacts with the dipolariphile styrene in a 1,3-dipolar cycloaddition reaction.The benzonitrile oxide is termed 1,3-dipole because of one of the resonance forms in which the formal position of the positive and negative charges are 1,3 with respect to one another. However, the term 1,3 does not directly relate to the position of the charges themselves but to the position of the bonding atoms in the dipolar molecule.In this cycloaddition reaction, the dipole atoms in position 1 and 3 of the benzonitrile oxime (LUMO) bind to the styrene (HOMO) to form diphenylisoxazoline. Benzonitrile oxime contributes four electrons to the system two electrons from the bond and two non-bonding electrons from the oxygen or nitrogen. On the other hand, the dipolariphile styrene contributes further two electrons. In total 4 + 2 an electronically allowed cycloadattion in which all 4 +2 electrons are in the ground state (termal).Depending of the spacial orientation of the styrene in solution, there are two theoretical products possible3,4 regioisomer3,5 regioisomerThe reaction therefore allows 5-membered rings synthesis, proceeding with high stereospecificity. The study of spectra data such as infrared and 1H-NMR allows not only the underpination of the final product but also helps to typeset the regioselectivity of the reaction.Infra-red SpectraBy evaluation of the spectrum of diphenylisoxazoline, one can confirm the product synthesised. The peaks mentioned in the results session of this paper are indeed in accordance to the 3,5-diphenyl-2-isoxazoline structure.By comparison to the previous oxime spectrum, it is obvious the absence of the OH- group and the formation of a C-O bond. The sp2 hybrydised C-H stretches are also seen in the diphenylisoxazoline spectrum.Diastereostopic Systems and 1H-NMR SpectraDiastereostopic groups are not equivalent and have unlike chemical shits in NMR.A pair of hydrogens located in a carbon atom adjacent to a stereocenter is expected t o be diastereostopic.Diastereostopic protonsAccording to Pavia et al, in some compounds with diastereostopic hydrogens, the chemical shifts of Ha and Hb are different and the peaks split each other into doublet of doublets (2Jab).In this case of 3,5-diphenyl-2-isoxazoline, the adjacent proton Hc shows large differences between the vicinal bring togethers between ac (3Jac) and bc (3Jbc).Refering to NMR results in the results section, the geminal coupling constant between hydrogen 8 and 7 is large. Therefore, the presence of the diastereostopic hydrogens is confirmed as the geminal coupling depends upon the bond angle between both protons. In convention the smaller the angle the larger the coupling constant.H8 at CD 2J8,7 = 16.4 Hz and H7 at CD 2J7,8 = 16.4 HzHowever, the question remains Which is the final product 3,5-diphenyl-2-isoxazoline or 3,4-diphenyl-2-isoxazoline?By using an H-NMR prognosticator, one can estimate the difference in chemical shifts between the diastereostopic and adjacent protons in both compounds.3,5-diphenyl-2-isoxazoline or 3,4-diphenyl-2-isoxazoline?The use of an H-NMR predictor will help to understand the final product and its regioselectivity.Spectrum Prediction of H-NMR spectrum of 3,5-diphenylisoxazoline. See referencesSpectrum Prediction of H-NMR spectrum of 3,4-diphenylisoxazoline. See referencesAs one can see, the chemical shifts predicted for the 3,5-diphenyl product in respect to diastereostopic hydrogens and the methine hydrogens are 3ppm and 6ppm respectively. On the other hand, the chemical shifts for the 3,4-diphenyl product in respect to diastereostopic hydrogens and the methine hydrogens are 5ppm and 4.5ppm respectively.The above values for the 3,5-diphenyl product are in completion relation to the ones in the results section and hence the final product is the 3,5-diphenyl-2-isoxazoline.For the 3,5-diphenyl-2-isoxazoline, the vicinal coupling are in accordance to the structure of the this regio-isomer.H8 at CD 3J8, 6 = 8.4 HzH7 at CD 3J7,6 = 11.2 HzH6 at CE 3J6,7 = 11.2 Hz, 3J6,8 = 8.4 HzThe vicinal coupling constant depends upon the dihedral angle between the nuclei. As such, the 3,4-diphenyl product would have very different values.Furthermore, by looking at the structure of both compounds one could say that the 3,4-diphenyl product allows more steric hindrance than the 3,5-diphenyl product.Diazomethane and OzoneOzone and diazomethane both behave as 1,3-dipoles.Their reactions with styrene also yields 5-membered rings.Ozone with StyreneResonance forms of ozone as 1,3-dipolesDiazomethane with StyreneConclusionBoth reactions were successful and the products characterized. The regioselectivity of the isoxazoline was analysed by H-NMR spectrum and the product determined to be 3,5-diphenylisoxazoline.Melting stratum ranges could have helped to determine the purity of the samples.In order to further understand the factors contributing to the regioselectivity product of the 1,3-dipolar cycloadditi on reaction, molecular modelling software could be used to determine energy differences between the products and transition states and whether the reaction is thermodynamically or kinetically favoured or both.In conjunction with frontier orbital of both HOME and LUMO and vice versa of the reagents, one could determine the reason for one product being more favoured than other.Nevertheless, the reaction of styrene with 1,3-dipolar benzaldehyde oxime yields 3,5-diphenyl-2-isoxazoline